Utilization of Carbohydrates in Bamboo Shoots (Dendrocalamus asper) As an Alternative Media for the Growth of Candida albicans fungus

Bastian; Firna Nuha; Maria Ulva; Rossa Veronneca

doi:10.31851/sainmatika.v20i1.11303

Authors

Bastian IKesT Muhamamdiyah Palembang
Kamilatun 4Medical Laboratory Techology Program, Institute Of Health Sciences and Techology Muhammadiyah Palembang, Indonesia
Maria Ulva 4Medical Laboratory Techology Program, Institute Of Health Sciences and Techology Muhammadiyah Palembang, Indonesia
Rossa Veronneca 4Medical Laboratory Techology Program, Institute Of Health Sciences and Techology Muhammadiyah Palembang, Indonesia

DOI:

https://doi.org/10.31851/sainmatika.v20i1.11303

Keywords:

Alternative Media, Bamboo Shoots, Candida albicans

Abstract

Candidiasis disease caused by the fungus Candida albicans is a health problem in Indonesia. The chronic nature of the disease gets worse if it is accompanied by a fungal infection, which usually goes undiagnosed and untreated. Culture techniques can be used to identify the fungus Candida albicans. Mushroom culture in the laboratory requires a medium containing nutrients such as a carbohydrate source and a nitrogen source for growth. These nutrients can be found in bamboo shoots which contain high carbohydrates, protein, fat, vitamins, and crude fiber. So that bamboo shoots can be used as raw material for alternative mushroom growth media. The purpose of this study was to utilize bamboo shoots as a medium for the growth of Candida albicans herbal medicine. This research included collecting bamboo shoots, making rice bran media, inoculation of Candida albicans fungus on bamboo shoots media, and observing fungal growth. Candida albicans fungal colonies were confirmed by macroscopic and microscopic observations. The results showed that the fungus Candida albicans grew on both media, namely Bamboo Shoots Flour Media and Sabouraud Dextrose Agar (SDA) media at 37^oC for 48 hours. The conclusion of this study is that bamboo shoots media can be used as a medium for fungal growth. Bamboo shoots media can be used as an alternative medium for synthetic media to grow Candida albican

References

Basarang, M., & Rianto, R. (2018). Pertumbuhan Candida sp dan Aspergillus sp dari Bilasan Bronkus Penderita Tuberkulosis Paru pada Media Bekatul Growth of Candida sp and Aspergillus sp from Bronchoscopy Pulmonary Tuberculosis Patients on Bran Media. Ilmu Alam Dan Lingkungan, 9(18), 80.

Danela, S., Gede, L. S., & Ariami, P. (2019). Kacang Kedelai Sebagai Media Alternatif Pertumbuhan Bakteri Pseudomonas Aeruginosa. Jurnal Analis Medika Biosains (JAMBS), 6(1).

Duarsa, G. W. D. P., & Dwija, I. B. N. P. D. (2020). Prevalensi Candica Albicans Pada Sputum Pasien Tb Dan Tb-Hiv Di Instalasi Mikrobiologi Klinik Rumah Sakit Umum Pusat Sanglah Denpasar. Jurnal Medika Udayana, 9(3), 5–10.

Fadli, A. (2017). Media alami untuk pertumbuhan jamur Candida albicans penyebab kandidiasis dari tepung biji kluwih (Artocarpus communis). Jurnal Kesehatan Prima, 11(2), 158–170.

Junaedi, W. R., Suliati, Mutiarawati, D. T., & Endrarini, L. H. (2022). Media Alternatif Agar Jagung untuk Identifikasi Candida albicans. 12(2), 188–191.

Marchamtia Sarah Nur Awalia Fajari, & Qurrohman, M. T. (2021). Efektivitas Variasi Konsentrasi Tepung Talas Sebagai Media Alternatif Pertumbuhan Jamur Candida aalbicans. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 2(2), 185–197.

Mulyati, Jannah, S. E., & Wahyuningsih, R. (2019). Pembentukan Germ Tube Candida albicans dan Candida tropicalis pada Media Putih Telur. 35(2), 60–64.

Nadiarta, P. A. (2017). Pertumbuhan Bakteri Saluran Pencernaan Pada Media Fermentasi Rebung Bambu Tabah (Gigantochloa nigrociliata BUZE-KURZ). Jurnal Rekayasa Dan Manajemen Agroindustri, 5(2), 10–16.

Nadziroh, D. U., Candra, N., & Setiawan, E. (2018). Aktivitas Antifungi Air Perasan Syzygium Polyanthum Terhadap Candida albicans. Journal Cis-Trans (JC-T), 2(2), 13–19.

Naim, N., Arifuddin, M., Hurustiaty, & Hasan, Z. A. (2020). Efektifitas Berbagai Variasi Konsentrasi Bekatul Terhadap Pertumbuhan Candida albicans. Jurnal Media Analis Kesehatan, 11(1), 47–55.

Octaviani, M., & Fadila. (2018). Uji Aktivitas Antijamur Sari Buah Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap Jamur Candida albicans. Jurnal Katalisator, 3(2), 125–133.

Prayekti, Endah; Lukiyono, Y. T. (2022). Penggunaan Tepung Ampas Tahu Untuk Media Pertumbuhan Candida albicans dan Candida sp. 3(2), 170–183.

Pujawati, Sukma, R., Mamat, R., Ai, D., Rahayu, & Gustira, I. (2019). Uji Efektivitas Ekstrak Serai Dapur (Cymbopogon Citratus (Dc.) Stapf) Terhadap Pertumbuhan Candida Albicans Metode Makrodilusi. Jurnal Riset Kesehatan Poltekkes Kemenkes Bandung, 11(2), 267–273.

Rahmawati, A. A. (2021). Rebung Bambu Sebagai Alternatif Fitohormon Dalam Memacu Pertumbuhan Tunas, Pada Benih Dorman. Biofarm: Jurnal Ilmiah Pertanian, 17(1), 36–39.

Santri, N. F. (2017). Identifikasi Candida albicans pada Urine Ibu Hamil yang Berkunjung Di RSUD Labuang Baji Makassar. 24–26.

Septadiya, O., & Syadi, Yunan Kholifatuddin Isworo, J. T. (2018). Kadar Serat Dan Sifat Sensoris Snack Bar Dengan Penambahan Tepung Rebung Ampel ( Bambusa vulgaris ). Universitas Muhammadiyah Semarang, 73, 12–14.

Sundari, S., Wisrakarmila, W., & Faizah, F. (2021). Pemanfaatan Biji Mangga Arum Manis (Mangifera indica L.) Sebagai Media Alternatif Pertumbuhan Candida albicans dan Aspergillus sp. Jurnal Pengelolaan Laboratorium Pendidikan, 3(1), 14–17.

Tamam, B. (2019). Potensi Kacang Kedelai Sebagai Media Alternatif Pertumbuhan Jamur Candida albicans. Journal of Chemical Information and Modeling, 53(9), 1689–1699.

Wahyuningsih, S. (2019). Pengaruh Konsentrasi Enzim α – Amilase pada Hidrolisis Pati Labu Jepang (Kabocha). CHEESA: Chemical Engineering Research Articles, 2(1), 26. https://doi.org/10.25273/cheesa.v2i1.4433

Yuniliani, D., Wilson, W., & Isworo, J. T. (2018). Pemanfaatan Kacang Merah ( Phaseolus vulgaris L .) Sebagai Media Alternatif Terhadap Pertumbuhan Trichophyton sp. 1, 28–33.