Aktifitas Antifungi Kitosan Hasil Hidrolisis Enzimatik Terhadap Penyakit Antraknosa

Authors

  • Yadi Suryadi ICABIOGRAD_IAARD Bogor
  • Dwiningsih Susilowati Lab. Mikrobiologi dan Biokimia, BB Biogen
  • I Made Samudra Lab. Mikrobiologi dan Biokimia, BB Biogen

DOI:

https://doi.org/10.31851/sainmatika.v16i2.2826

Keywords:

anthracnose, chitosan, C.gloeosporioides, chitinase

Abstract

Anthracnose disease caused by Colletotrichum gloeosporioides is one of an important fungal pathogen on mango fruits during the pre harvest or post harvest stage. The use of chitosan as an alternative method to control the disease is necessary due to its biodegradable, biocompatible and non-toxic effect. Chitosan tripolyphosphate (C-TPP) showed higher inhibition to C. gloeosporioides compared with that of low molecular weight (LMW) chitosan as affected by its particle size. The best of disease inhibitory activity was revealed by C-TPP B [0.2% LMW : 0.1% TPP; (5: 2)] and C [0.2% LMW: 0:08% TPP; (5: 1)] treatments based on  in vitro and in vivo test. Because of the concentration of chitosan and TPP may affect to particle structure, hence the C-TPP formation needs further to be optimized so that the size is getting smaller and stable.

Author Biography

Yadi Suryadi, ICABIOGRAD_IAARD Bogor

Biokimia

References

Ali, A., Zahid, N., Manickam, S., & Siddiqui, Y. (2013). Effectiveness of submicron chitosan dispersions in controlling anthracnose and maintaining quality of dragon fruit. Postharvest Biology and Technology, 86, 147–153.

Brugnerotto, J., Lizardi, J., Goycoolea, F. M., Argüelles-Monal, W., Desbrières, J., & Rinaudo, M. (2001). An infrared investigation in relation with chitin and chitosan characterization. Polymer, 42(8), 3569–3580. https://doi.org/10.1016/S0032-3861(00)00713-8

Gonzalez-Aguilar, G. A., Celis, J., Sotelo-Mundo, R. R., De La Rosa, L. A., Rodrigo-Garcia, J., & Alvarez-Parrilla, E. (2008). Physiological and biochemical changes of different fresh-cut mango cultivars stored at 5°C. International Journal of Food Science and Technology, 43(1), 91–101. https://doi.org/10.1111/j.1365-2621.2006.01394.x

Goy, R. C., Britto, D. De, & Assis, O. B. G. (2009). A review of the antimicrobial activity of chitosan. Polimeros:Ciencia et Tecnologia. 19(3), 241-247.https://doi.org/10.1590/S0104-14282009000300013

Hewajulige, I., Sultanbawa, Y., & Wijeratnam, S. W. (2009). Mode of action of chitosan coating on anthracnose disease control in papaya. Phytoparasitica. 37, 437–444. https://doi.org/10.1007/s12600-009-0052-5

Hu, M., Yang, D., Huber, D. J., Jiang, Y., Li, M., Gao, Z., & Zhang, Z. (2014). Reduction of postharvest anthracnose and enhancement of disease resistance in ripening mango fruit by nitric oxide treatment. Postharvest Biology and Technology, 97, 115–122. https://doi.org/10.1016/j.postharvbio.2014.06.013

Ing, L. Y., Zin, N. M., Sarwar, A., & Katas, H. (2012). Antifungal activity of chitosan nanoparticles and correlation with their physical properties. International Journal of Biomaterials, 2012, 1–9. https://doi.org/10.1155/2012/632698

Palma-Guerrero J., Jansson H.B., Salinas, J., & Liorca, L.V.L. (2008). Effect of chitosan on hyphal growth and spore germination of plant pathogenic and biocontrol fungi, J. of Applied Microbiology 104, 541–553. https://doi.org/10.1111/j.13652672.2007.03567.x

Jovanović, G. D., Klaus, A. S., & Nikšić, M. P. (2016). Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish. Revista Argentina de Microbiología, 48(2), 128–136. https://doi.org/10.1016/j.ram.2016.02.003

Kumar, A.B.V., Varadaraj, M.C., Gowda, L.R., & Tharanathan, R,N. (2007). Low molecular weight chitosan-preparation with the aid of pronase, characterization and their bactericidal activity towards Bacillus cereus and Escherichia coli. Biochimica et Biophysica Acta. 1770: 495-505. (2007).

Lauzardo, A.N.H., Del Valle, M.G.V., Castelan, L.V., Giorgana, G.E.M, & Sánchez, M.G.G. (2009). Effect of chitosan on three isolates of Rhizopus stolonifer obtained from peach, papaya and tomato, Fruits 65(4), 245–253. https://doi.org/10.1051/fruits/2010020

Li, X.F., Feng, X.Q., Yang, S., Wang, T.P., & Su, Z.X. (2008). Effects of molecular weight and concentration of chitosan on antifungal activity against Aspergillus niger. Iranian Polymer Journal, 17(11), 843–852.

Mardliyati, E., Sjaikhurrizal E.M, Damai R.S. 2012. Sintesis nanopartikel kitosan-trypoly phosphate dengan metode gelasi ionik: Pengaruh konsentrasi dan rasio volume terhadap karakteristik partikel. Prosiding Pertemuan Ilmiah Ilmu Pengetahuan dan Teknologi Bahan; 2012 (Okt 3); Serpong, Indonesia. Serpong (ID): BPPT. hlm 90-93.

Sharma, M & Kulshrestha, S. (2018). Colletotrichum gloeosporioides : An anthracnose causing pathogen of fruits and vegetables, Biosciences Biotechnology Research Asia,12 (2),1-14. https://doi.org/10.13005/bbra/1776

Mulyaningtyas, D., A, Purwantisari, S., Kusdiyantini, E., & Suryadi, Y. (2016). Produksi kitosan secara enzimatik oleh Bacillus firmus E65 untuk pengendalian penyakit antraknosa pada buah mangga (Mangifera Indica L.). Jurnal Biologi, 5(4), 8–17.

Suryadi, Y., Priyatno, T. P., Samudra, I. M., Susilowati, D. N., Patricia, & Irawati, W. (2013). Karakterisasi dan identifikasi isolat bakteri endofitik penghambat jamur patogen Padi. Buletin Plasma Nutfah, 19(1), 25–32.

Suryadi, Y., Priyatno, T. P., Samudra, I. M., Susilowati, D., & Sriharyani, T. S. (2017). Control of anthracnose disease ( Colletotrichum gloeosporioides) using nano chitosan hydrolyzed by chitinase derived from Burkholderia cepacia isolate E76, J. Agrobiogen 13(2), 111–122.

Toharisman. (2015). Purification and characterization of a thermostable chitinase from Bacillus licheniformis Mb-2. World Journal of Microbiology and Biotechnology, 21, 733–738. https://doi.org/10.1007/s11274-004-4797-1

Zhong, Z., Chen, R., Xing, R., Chen, X., Liu, S., Guo, Z., & Li, P. (2007). Synthesis and antifungal properties of sulfanilamide derivatives of chitosan. Carbohydrate Research. 342 (16): 2390–2395. https://doi.org/10.1016/j.carres.2007.07.015

Downloads

Published

2019-07-09