Pengaruh Umur Kematian Tanaman Sumber Inokulum Banana Bunchy Top Virus Terhadap Efisiensi Penularannya


  • Titi Tricahyati Sriwijaya University
  • Suparman Suparman Sriwijaya University
  • Chandra Irsan Sriwijaya University



Keywords, BBTV, Pentalonia nigronervosa, dead banana plant


BBTV is one of the important diseases in banana plants, the transmission of BBTV can be carried out by Pentalonia nigronervosa. In some banana plantations infected plants are usually cut down because they are considered unable to produce optimal production. The felled plants will then be placed around healthy plantations, this situation is thought to have the potential as a place for P. nigronervosa to temporarily live and spread the virus to other healthy plants. This study aims to determine the ability of dead plants that host P. nigronervosa to re-transmit the virus to healthy plants. The study was conducted using a Randomized Block Design (RAK) with six treatments on the day of death of infected plants, namely, 0, 5, 10, 15, 20, and 25 days, with five replications. From the results of the study, it was found that plants infected by BBTV still had potential to transmit the virus to healthy plants through the P. nigronervosa. Transmission of BBTV from dead plants on the fifth day resulted in the highest average of BBTV infection. The incubation period of virus in plants ranged from 30-53 days after inoculation. From this research, it is hoped that farmers will pay more attention to thoroughly eradicate banana plants infected by BBTV, removed all plant and suckers thoroughly together with their corms.


Borth, W., Perez, E., Cheah, K., Chen, Y., Xie, W. S., Gaskill, D., Khalil, S., Sether, D., Melzer, M., Wang, M., Manshardt, R., Gonsalves, D., & Hu, J. S. (2011). Transgenic banana plants resistant to Banana Bunchy top virus infection. Acta Horticulturae, 897, 449–458.

Calvo, D., & Fereres, A. (2011). The performance of an aphid parasitoid is negatively affected by the presence of a circulative plant virus. BioControl, 56(5), 747–757.

Dale, J. L. (1987). Banana bunchy top: An economically important tropical plant virus disease. Advances in Virus Research, 33(C), 301–325.

Damasco, O. P., Cueva, F. M. Dela, Descalsota, J. C., & Tayobong, R. R. P. (2019). Gamma Radiation and In Vitro Induced Banana Bunchy Top Virus ( BBTV ) Resistant Mutant Lines of Banana cv ‘ Lakatan ’ ( Musa sp ., AA ). Philippine Journal of Science, 149(Psa 2018), 159–173.

Department of Health. (2016). The biology of Musa L (banana) (Issue January, pp. 1–77).

El-Dougdoug, Kh. A. (2011). Management of viral disease in banana using certified and virus tested plant material. African Journal of Microbiology Research, 5(32), 5923–5932.

Foottit, R. G., Maw, H. E. L., Pike, K. S., & Miller, R. H. (2010). The identity of Pentalonia nigronervosa Coquerel and P. caladii van der Goot (Hemiptera: Aphididae) based on molecular and morphometric analysis. ZOOTAXA, 115(2884), 25–38.

Galán Saúco, V. (2020). Broad overview of the subtropical banana industry. Acta Horticulturae, 1272, 1–12.

Grigoras, I., Vetten, H. J., Commandeur, U., Ziebell, H., Gronenborn, B., & Timchenko, T. (2018). Nanovirus DNA-N encodes a protein mandatory for aphid transmission. Virology, 522(May), 281–291.

Hafner, G. J., Harding, R. M., & Dale, J. L. (1995). Movement and transmission of Banana bunchy top virus DNA component one in bananas. Journal of General Virology, October.

Halbert, Susan E., Baker, Carlye A. (2015.) Banana bunchy top virus and its vector Pentalonia nigronervosa ( Hemiptera : Aphididae )1. Pathology Circular, 417, 1-7

Haq, I. (2015). Aseptic Growth Attributes of PEG-Stressed and BBTV-Infected Plants of Banana. International Journal of Science and Research, 4(6), 76–80.

Hooks, C. R. R., Wright, M. G., Kabasawa, D. S., Manandhar, R., & Almeida, R. P. P. (2008). Effect of banana bunchy top virus infection on morphology and growth characteristics of banana. Annals of Applied Biology, 153(1), 1–9.

Hu, J. S., Wang, M., Sether, D., Xie, W., & Leonhardt, K. W. (1996). Use of polymerase chain reaction (PCR) to study transmission of banana bunchy top virus by the banana aphid (Pentalonia nigronervosa). Annals of Applied Biology, 128(1), 55–64.

Jacobsen, K., Omondi, B. A., Almekinders, C., Alvarez, E., Blomme, G., Dita, M., Iskra-Caruana, M. L., Ocimati, W., Tinzaara, W., Kumar, P. L., & Staver, C. (2019). Seed degeneration of banana planting materials: strategies for improved farmer access to healthy seed. Plant Pathology, 68(2), 207–228.

Jeger, M. J. (2020). The epidemiology of plant virus disease: Towards a new synthesis. Plants, 9(12), 1–50.

Jekayinoluwa, T., Tripathi, J. N., Dugdale, B., Obiero, G., Muge, E., Dale, J., & Tripathi, L. (2021). Transgenic Expression of dsRNA Targeting the Pentalonia nigronervosa acetylcholinesterase Gene in Banana and Plantain Reduces Aphid Populations. Plants, 10(613), 1-20. 3.

Jekayinoluwa, T., Tripathi, L., Nath, J., Otang, V., George, N., Edward, O., & James, M. (2020). RNAi technology for management of banana bunchy top disease. Food and Energy Security, August, 1–15.

Jones, R. A. C. (2021). Global plant virus disease pandemics and epidemics. Plants, 10(2), 1–41.

Kakati, N., Dutta, P., Das, P., & Nath, P. D. (2018). Compatibility of Entomopathogenous Fungi with Commonly used Insecticides for Management of Banana Aphid transmitting Banana bunchy Top virus ( BBTV ) in Assam Banana Production System. International Journal of Current Microbiology and Applied Sciences, 7(11), 2507-2513.

King, A., Adams, M., Lefkowitz, E., & Carstens, E. (2012). Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses (A. M. Q. King, M. J. Adams, E. B. Carstens, & E. J. Lefkowitz (eds.)). Elsevier Academic Press.

King, A. M. Q., Adams, M. J., Carstens, E. B., & Lefkowitz, E. J. (2012). Virus Taxonomy (A. M. Q. King, M. J. Adams, E. B. Carstens, & E. J. Lefkowitz (eds.)). Academic Press is an imprint of Elsevier.

Kiritani, K., & Su, H. J. (1999). Papaya ring spot, banana bunchy top, and citrus greening in the Asia and Pacific region: Occurrence and control strategy. Japan Agricultural Research Quarterly, 33(1), 23–30.

Magnaye, L. V., & Valmayor, R. V. (2003). BBTV, CMV and other viruses affecting banana in Asia and the Pacific. FTTC Extension Bulletins, 1–6.

Mathers, T. C., Mugford, S. T., Hogenhout, S. A., & Tripathi, L. (2020). Genome sequence of the banana aphid, Pentalonia nigronervosa coquerel (hemiptera: Aphididae) and its symbionts. G3: Genes, Genomes, Genetics, 10(12), 4315–4321.

Molina, a B., Roa, V. N., Carpio, a T., & Joven, J. E. a. (1998). Managing banana and citrus diseases. Proceedings of a Regional Workshop on Disease Management of Banana and Citrus through the Use of Disease-Free Planting Materials Held in Davao City, Philippines - 14-16 October 1998, October, 163.

Molina, A. B., Sinohin, V. G. O., Dela Cueva, F. M., Esguerra, A. V., Crucido, S. S., Vida, E., Temanel, B. E., Anit, E. A., & Eusebio, J. E. (2009). Managing banana bunchy top virus in smallholdings in the Philippines. Acta Horticulturae, 828, 383–388.

Montong, V. B., Maramis, R. T. D., Pelealu, J., & Christina, L. (2015). Involvement Of Insects In The Transmission Of Banana Blood Disease. 3(1), 41–44.

Nelson, S. C. (2004). Banana bunchy top : detailed signs and symptoms. Knowledge Creation Diffusion Utilization, 1–22.

Niyongere, C., Losenge, T., Ateka, E. M., Ntukamazina, N., Ndayiragije, P., Simbare, A., Cimpaye, P., Nintije, P., Lepoint, P., & Blomme, G. (2013). Understanding banana bunchy top disease epidemiology in Burundi for an enhanced and integrated management approach. Plant Pathology, 62(3), 562–570.

Nurhayati, E. (2003). Incidence of Banana Bunchy Top Disease In West Java, Indonesia. Jurnal Perlindungan Tanaman Indonesia, 9(2), 81–86.

Pekin, J. (2015). Final Report Banana Bunchy Top Virus - Phase 2. Horticulture Innovation Australia Limited.

Picaza, A. R., & Doloriel, D. M. (2018). An Agent-Based Computer Simulation on Banana Bunchy Top Disease. International Journal of Environment, Agriculture and Biotechnology, 3(2), 637–640.

Pinili, M. S., Nyoman, D., Ana, N., Suastika, G., & Natsuaki, K. T. (2011). Molecular Analysis of Banana bunchy top virus First Isolated in Bali, Indonesia. J. Agric. Sci.. Tokyo Univ. Agric, 56(2), 125–134.

Pinili, M. S. P., Agashima, I. N., Izon, T. O. D., & Atsuaki, K. T. N. (2013). Cross-Transmission and New Alternate Hosts of Banana bunchy top virus. Tropical Agriculture and Development, 57(1), 1–7.

Pinto, L. J., & Kraft, S. K. (2014). Integrated pest management training manual. Integrated Pest Management for Citrus, University of California, 24–119.

Pitan, O. O. R., & Filani, C. O. (2013). Assessment of Insect Spectrum, and Insect-Induced Damage at Different Growth Stages of Cucumber (Cucumis sativus L.) In A Rainforest Transition Zone Of Nigeria. Annals of Tropical Research, 68, 60–68.

Ramakrishna, A., Reddy, B. D., & Khaire, P. B. (2020). Integrated Disease Management Strategies of Banana Diseases. 1(05), 26–30.

Ranasingh, N. (2007). Field Diagnosis and Management of Banana Bunchy Top Disease. Orisa Review, 78–80.

Robson, J. D., Wright, M. G., & Almeida, R. P. P. (2007). Effect of imidacloprid foliar treatment and banana leaf age on Pentalonia nigronervosa (Hemiptera, aphididae) survival. New Zealand Journal of Crop and Horticultural Science, 35(4), 415–422.

Shahrukh, I.-H., Um-E-Aiman, Saifullah, K., Nazia, P., Madiha, F., & Muhammad, U. D. (2014). Certain growth related attributes of bunchy top virus infected banana under ex-vitro conditions. African Journal of Biotechnology, 13(18), 1876–1882.

Shiragi, M. H. K., Baque, M. a, & Nasiruddin, K. M. (2010). Eradication of Banana Bunchy Top Virus (BBTV) Through Meristem Culture of Infected Plant Banana cv. Sabri. Horticulture Environment and Biotechnology, 51(3), 212–221.

Singh, V., Adil, S., & Quraishi, A. (2022). Elimination of BBTV via a systemic in vitro electrotherapy approach. Journal of Virological Methods, 300(September 2021), 1-13.

Staton, T., Walters, R., Smith, J., Breeze, T., & Girling, R. (2021). Management to promote flowering understoreys benefits natural enemy diversity, aphid suppression and income in an agroforestry system. Agronomy, 11(4), 1-17.

Stechmann, D. â€H, & Völkl, W. (1990). A preliminary survey of aphidophagous insects of Tonga, with regards to the biological control of the banana aphid. Journal of Applied Entomology, 110(1–5), 408–415.

Su, H.-J., Hwang, A.-S., Lee, S.-Y., & Chao, C.-P. (2007). Conservation, Disease-indexing and Utilization of Pathogen-free Citrus and Banana Genetic Resources in Taiwan. In The Conservation and Utilization of Tropical/Subtropical Plant Genetic Resources.

Sutrawati, M., & Ginting, S. (2020). First report of banana bunchy top disease on banana in Bengkulu. Plant Disease, 3(2), 82–87.

Thomas, J. E. (2008). Banana Bunchy Top Virus. Encyclopedia of Virology, 272–279.

Thomas, J. E. (2010). Technical Guidelines for the Safe Movement of Musa Germplasm. In Bioversity International (Issue 20).

Timchenko, T., de Kouchkovsky, F., Katul, L., David, C., Vetten, H. J., & Gronenborn, B. (1999). A Single Rep Protein Initiates Replication of Multiple Genome Components of Faba Bean Necrotic Yellows Virus, a Single-Stranded DNA Virus of Plants. Journal of Virology, 73(12), 10173–10182.

Tongo, M. L. F., Muengula, M., Zinga, I., Kalonji, A., Iskra-Caruana, M. L., & Bragard, C. (2014). Occurrence and Distribution of Banana bunchy top virus Related Agro-Ecosystem in South Western, Democratic Republic of Congo.

Yahya, Z. G. A. (2019). Plant virus identification and virus-vector-host interactions. Retrieved from ORCID Digital Dissertation. (ORCID 0000-0002-7833-1542)