Tingkat Patogenisitas Bakteri Vibrio sp. Strain IS8 Yang Diinfeksi Pada Kerang Batik (Ruditapes philippinarum) Menggunakan  Metode In Vitro

Ciptaning Weargo Jati; Huriyatul Fitriyah Noor; Juli Nursandi; Rahma Mulyani

doi:10.31851/jipbp.v18i2.14567

Authors

Ciptaning Weargo Jati Universitas Lampung
Huriyatul Fitriyah Noor
Juli Nursandi
Rahma Mulyani

DOI:

https://doi.org/10.31851/jipbp.v18i2.14567

Keywords:

brown ring disease, manila clam, ruditapes philippinarum, Vibrio tapetis, Penyakit Cincin Coklat, Kerang Batik, Ruditapes philippinarum

Abstract

Kerang, khususnya jenis Ruditapes philippinarum, memiliki nilai ekonomis tinggi di Prancis dan banyak wilayah lainnya. Meskipun teknologi budidaya dan pengendalian penyakit berkembang pesat, penyakit musiman seperti Brown Ring Disease (BRD) atau penyakit cincin coklat tetap menjadi tantangan dalam budidaya R. philippinarum. Penyakit ini disebabkan oleh Vibrio tapetis, yang memiliki dampak patogenik yang signifikan pada kerang. Penelitian ini fokus pada pengujian tingkat patogenisitas V. tapetis strain IS8 pada kerang batik (R. philippinarum). Melalui uji aderensi dan fagositosis, kami mengevaluasi kemampuan bakteri ini untuk menyebabkan efek aderen pada hemosit kerang dan aktivitas fagositosis. Hasilnya menunjukkan bahwa V. tapetis strain IS8 memiliki tingkat patogenisitas yang sangat tinggi, dengan kemampuan aderensi dan aktivitas fagositosis yang lebih tinggi dari kontrol positif. Kesimpulan dari penelitian ini adalah bahwa V. tapetis strain IS8 mampu menginduksi penyakit cincin coklat pada kerang batik dan memiliki dampak patogenik yang signifikan. Pengetahuan lebih lanjut tentang mekanisme infeksius bakteri ini diperlukan untuk meningkatkan pemahaman tentang peran mereka dalam budidaya kerang di masa depan.

Clams, particularly Ruditapes philippinarum, hold significant economic value in France and various other regions. Despite rapid advancements in culture technology and disease control, seasonal diseases such as Brown Ring Disease (BRD) continue to pose a challenge in the cultivation of R. philippinarum. This ailment is attributed to Vibrio tapetis, exhibiting a substantial pathogenic impact on clams. This study concentrates on assessing the pathogenicity level of V. tapetis strain IS8 in the Manila clam (R. philippinarum). Through adhesion and phagocytosis assays, we evaluate the capability of V. tapetis strain IS8 to induce adhesion effects on clam hemocytes and phagocytic activity. The results demonstrate that V. tapetis strain IS8 exhibits a remarkably high level of pathogenicity, surpassing the positive control in both adhesion and phagocytosis assays. In conclusion, this research establishes that V. tapetis strain IS8 can induce Brown Ring Disease in Manila clams, showcasing a significant pathogenic impact. Further comprehension of the infectious mechanisms of this bacterium is essential for enhancing our understanding of their role in clam cultivation in the future.

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