Efikasi Vaksin Booster Streptococcus agalactiae pada Induk Ikan Nila Terhadap Imunitas Maternal untuk Pencegahan Streptococcosis
DOI:
https://doi.org/10.31851/jipbp.v17i2.9277Keywords:
imunitas maternal, induk, nila, transfer, vaksin boosterAbstract
Bakteri Streptococcus agalactiae merupakan patogen utama yang menyerang ikan nila mulai fase benih hingga dewasa. Tujuan penelitian ini yaitu untuk menguji efikasi vaksin booster S. agalactiae di induk ikan nila sebelum pemijahan kedua terhadap imunitas maternal untuk pencegahan streptococcosis. Vaksin gabungan sediaan sel utuh dan produk ekstraselular (ECP) S. agalactiae diinjeksi sebanyak 0,4 mL/kg induk ikan dengan perbandingan 50:50% (v/v) dari dosis penyuntikan, sedangkan kontrol diinjeksi dengan phosphate buffered saline (PBS). Vaksin diberikan ke induk ikan pada fase tingkat kematangan gonad dua (TKG 2). Perlakuan penelitian yaitu induk diinjeksi PBS (K), induk diinjeksi vaksin satu kali (A), dan induk diinjeksi vaksin booster setelah pemijahan pertama (B). Uji tantang benih dari setiap induk perlakuan dilakukan melalui perendaman S. agalactiae 107 CFU/mL selama 30 menit pada umur benih 5, 10, 15, dan 20 hari setelah menetas. Hasil penelitian menunjukkan terdapat perbedaan yang signifikan (P<0,05) pada total leukosit dan aktivitas fagositik induk ikan. Level antibodi dan lisozim induk, telur, dan benih dari perlakuan B signifikan lebih tinggi (P<0,05) dibandingkan dengan perlakuan induk lainnya. Daya tetas telur dari perlakuan induk B (94,52%) signifikan lebih tinggi (P<0,05) dari pada perlakuan induk lainnya. Nilai Relative percent Survival benih dari perlakuan induk B tidak berbeda signifikan (P>0,05) dengan perlakuan benih dari induk A pada hari ke-5 tetapi, signifikan lebih tinggi (P<0,05) pada hari ke-10 hingga 20 pascatetas (96,61-54,17%). Pemberian vaksin booster di induk ikan nila sebelum pemijahan kedua dapat menstimulasi peningkatan imunitas dan transfer imunitas maternal ke anaknya untuk pencegahan streptococcosis.References
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